Present Book entitled “A Practical Manual OnPlant Biotechnology” is according to ICAR Vth Deans Committee Recommendations and is for B.Sc. (H) Agriculture, For B.Sc. (H) Forestry, For B.Sc. (H) Horticulture, M.Sc (Genetics & Plant Breeding) and M. Sc (Molecular Biology & Biotechnology) students. It covers basic to advance techniques used in Plant Biotechnologyi.eBasic Laboratory set up of Plant Biotechnology Lab, Laboratory and Personal Safety, Equipment’s & Instruments used in Biotechnology lab, Embryo culture , Genomic DNA Isolation ,Quantitation of DNA, Agarose Gel Electrophoresis, Polymerase Chain Reaction (PCR), Separation of Proteins by S.D.S- P.A.G.E, Isolation of plasmid DNA , Transformation of competent cells of E.coli with plant transformation vectors and Detection of transgenes using PCR etc.